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Rational Design of and Mechanism Insight into an Efficient Antifreeze Peptide for Cryopreservation

Haishan Qi,Yihang Gao,Lin Zhang,Zhongxin Cui,Xiaojie Sui,Jianfan Ma,Jing Yang,Zhiquan Shu,Lei Zhang,

《工程（英文）》 doi: 10.1016/j.eng.2023.01.015

摘要： The development of effective antifreeze peptides to control ice growth has attracted a significant amount of attention yet still remains a great challenge. Here, we propose a novel design method based on an in-depth investigation of repetitive motifs in various ice-binding proteins (IBPs) with an evolution analysis. In this way, several peptides with notable antifreeze activity were developed. In particular, a designed antifreeze peptide named AVD exhibits ideal ice recrystallization inhibition (IRI), solubility, and biocompatibility, making it suitable for use as a cryoprotective agent (CPA). A mutation analysis and molecular dynamics (MD) simulations indicated that the Thr6 and Asn8 residues of the AVD peptide are fundamental to its ice-binding capacity, while the Ser18 residue can synergistically enhance their interaction with ice, revealing the antifreeze mechanism of AVD. Furthermore, to demonstrate the cryoprotection potential of AVD, the peptide was successfully employed for the cryopreservation of various cells, which demonstrated significant post-freezing cell recovery. This work opens up a new avenue for designing antifreeze materials and provides peptide-based functional modules for synthetic biology.

关键词： Antifreeze peptides     Evolution analysis     Ice recrystallization inhibition     Molecular dynamics simulation     Cryopreservation     Synthetic biology    

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Microstructure investigation of dynamic recrystallization in hard machining: From thermodynamic irreversibility

Binxun LI, Xinzhi ZHANG, Song ZHANG

《机械工程前沿（英文）》 2021年 第16卷 第2期   页码 315-330 doi: 10.1007/s11465-020-0612-4

摘要： The drastically changed thermal, mechanical, and chemical energies within the machined surface layer during hard machining tend to initiate microstructural alteration. In this paper, attention is paid to the introduction of thermodynamic potential to unravel the mechanism of microstructure evolution. First, the thermodynamic potential-based model expressed by the Helmholtz free energy was proposed for predicting the microstructure changes of serrated chip and the machined surface layer. Second, the proposed model was implemented into a validated finite element simulation model for cutting operation as a user-defined subroutine. In addition, the predicted irreversible thermodynamic state change in the deformation zones associated with grain size, which was reduced to less than 1 m from the initial size of 1.5 m on the machined surface, was provided for an in-depth explanation. The good consistency between the simulated results and experimental data validated the efficacy of the developed model. This research helps to provide further insight into the microstructure alteration during metal cutting.

关键词： thermodynamic irreversibility     Helmholtz free energy     microstructure evolution     dynamic recrystallization     hard milling    

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Chronic inhibition of cyclic guanosine monophosphate-specific phosphodiesterase 5 prevented cardiac fibrosisthrough inhibition of transforming growth factor β-induced Smad signaling

null

《医学前沿（英文）》 2014年 第8卷 第4期   页码 445-455 doi: 10.1007/s11684-014-0378-3

摘要：

Recent evidences suggested that cyclic guanosine monophosphate-specific phosphodiesterase 5 (PDE5) inhibitor represents an important therapeutic target for cardiovascular diseases. Whether and how it ameliorates cardiac fibrosis, a major cause of diastolic dysfunction and heart failure, is unknown. The purpose of this study was to investigate the effects of PDE5 inhibitor on cardiac fibrosis. We assessed cardiac fibrosis and pathology in mice subjected to transverse aortic constriction (TAC). Oral sildenafil, a PDE5 inhibitor, was administered in the therapy group. In control mice, 4 weeks of TAC induced significant cardiac dysfunction, cardiac fibrosis, and cardiac fibroblast activation (proliferation and transformation to myofibroblasts). Sildenafil treatment markedly prevented TAC-induced cardiac dysfunction, cardiac fibrosis and cardiac fibroblast activation but did not block TAC-induced transforming growth factor-β1 (TGF-β1) production and phosphorylation of Smad2/3. In isolated cardiac fibroblasts, sildenafil blocked TGF-β1-induced cardiac fibroblast transformation, proliferation and collagen synthesis. Furthermore, we found that sildenafil induced phosphorylated cAMP response element binding protein (CREB) and reduced CREB-binding protein 1 (CBP1) recruitment to Smad transcriptional complexes. PDE5 inhibition prevents cardiac fibrosis by reducing CBP1 recruitment to Smad transcriptional complexes through CREB activation in cardiac fibroblasts.

关键词： PDE5     cardiac fibrosis     TGF-β     CREB    

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Numerical simulation on flow of ice slurry in horizontal straight tube

Shengchun LIU,Ming SONG,Ling HAO,Pengxiao WANG

《能源前沿（英文）》 2021年 第15卷 第1期   页码 201-207 doi: 10.1007/s11708-017-0451-0

摘要： Numerical simulation on flow of ice slurry in horizontal straight tubes was conducted in this paper to improve its transportation characteristics and application. This paper determined the influence of the diameter and length of tubes, the ice packing factors (IPF) and the flow velocity of ice slurry on pressure loss by using numerical simulation, based on two-phase flow and the granular dynamic theory. Furthermore, it was found that the deviation between the simulation results and experimental data could be reduced from 20% to 5% by adjusting the viscosity which was reflected by velocity. This confirmed the reliability of the simulation model. Thus, two mathematical correlations between viscosity and flow velocity were developed eventually. It could also be concluded that future rheological model of ice slurry should be considered in three sections clarified by the flow velocity, which determined the fundamental difference from single-phase fluid.

关键词： ice slurry     horizontal tubes     numerical simulation     pressure drop     viscosity model    

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Application of Ice Pigging in a Drinking Water Distribution System: Impacts on Pipes and Bulk Water Quality

Yujing Huang,Zhiwei Chen,Guilin He,Yu Shao,Shuang Song,Feilong Dong,Tuqiao Zhang,

《工程（英文）》 doi: 10.1016/j.eng.2023.09.016

摘要： Ice pigging is an emerging technique for pipe cleaning in drinking water distribution systems. However, substantial confusion and controversy exist on the potential impacts of ice pigging on bulk water quality. This study monitored the microstructural features and composition of sediments and microbial community structures in bulk water in eight multimaterial Chinese networks. Chloride concentration analysis demonstrated that separate cleaning of pipes with different materials in complex networks could mitigate the risk of losing ice pigs and degrading water quality. The microstructural and trace element characterization results showed that ice pigs would scarcely disturb the inner surfaces of long-used pipes. The bacterial richness and diversity of bulk water decreased significantly after ice pigging. Furthermore, correlations were established between pipe service age, temperature, total iron and chloride concentrations, and the 15 most abundant taxa in bulk water, which could be used to guide practical ice pigging operations.

关键词： Ice pigging     Pipe cleaning     Drinking water distribution system     Bacterial community     Sediments    

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Purification of ice structuring protein complexes from winter wheat using Triton X-114 phase partitioning

Huaneng XU, Haiying CHEN, Weining HUANG,

《化学科学与工程前沿（英文）》 2009年 第3卷 第4期   页码 383-385 doi: 10.1007/s11705-009-0249-7

摘要： Ice structuring proteins (ISPs) isolated from the cold-acclimated plants have a great potential in improving the quality of frozen foods. The purification of ISP complexes from winter wheat was achieved using an aqueous two-phase system of Triton X-114. The highly reactive phenols were removed, and the ISP complexes remained in the aqueous phase after phase separation. The ISP complexes treated by this procedure retained higher thermal hysteresis activity than those treated by ammonium sulfate method. The phase separation technique provides a simple and mild way for removing phenols from ISP complexes.

关键词： separation technique     thermal hysteresis     cold-acclimated     purification     X-114    

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simultaneous anammox and denitrification system with short-term pyridine exposure: Process capability, inhibition

《环境科学与工程前沿（英文）》 2021年 第15卷 第6期 doi: 10.1007/s11783-021-1433-3

摘要：

• Short-term effect of the pyridine exposure on the SAD process was investigated.

关键词： Anammox     Inhibition     Metabolic pathway     Microbial community     Pyridine     SAD    

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ROOT EXUDATES FROM CANOLA EXHIBIT BIOLOGICAL NITRIFICATION INHIBITION AND ARE EFFECTIVE IN INHIBITING

《农业科学与工程前沿（英文）》 2022年 第9卷 第2期   页码 177-186 doi: 10.15302/J-FASE-2021421

摘要：

A range of plant species produce root exudates that inhibit ammonia-oxidizing microorganisms. This biological nitrification inhibition (BNI) capacity can decrease N loss and increase N uptake from the rhizosphere. This study sought evidence for the existence and magnitude of BNI capacity in canola ( Brassica napus). Seedlings of three canola cultivars, Brachiaria humidicola(BNI positive) and wheat ( Triticum aestivum) were grown in a hydroponic system. Root exudates were collected and their inhibition of the ammonia oxidizing bacterium, Nitrosospira multiformis, was tested. Subsequent pot experiments were used to test the inhibition of native nitrifying communities in soil. Root exudates from canola significantly reduced nitrification rates of both N. multiformis cultures and native soil microbial communities. The level of nitrification inhibition across the three cultivars was similar to the well-studied high-BNI species B. humidicola. BNI capacity of canola may have implications for the N dynamics in farming systems and the N uptake efficiency of crops in rotational farming systems. By reducing nitrification rates canola crops may decrease N losses, increase plant N uptake and encourage microbial N immobilization and subsequently increase the pool of organic N that is available for mineralization during the following cereal crops.

关键词： ammonia oxidizing microorganisms / biological nitrification inhibition / farming rotations / nitrogen cycling / nitrogen use efficiency    

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Inhibition character of crotonaldehyde manufacture wastewater on biological acidification

《环境科学与工程前沿（英文）》 2021年 第15卷 第6期 doi: 10.1007/s11783-021-1403-9

摘要：

• The inhibition of the main organic pollutions in CMW was demonstrated.

关键词： Crotonaldehyde manufacture wastewater     Biological acidification     Volatile fatty acids     Inhibition     Toxic units    

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Roles of integrin β3 cytoplasmic tail in bidirectional signal transduction in a trans-dominant inhibition

null

《医学前沿（英文）》 2016年 第10卷 第3期   页码 311-319 doi: 10.1007/s11684-016-0460-0

摘要：

We evaluated the roles of calpain cleavage-related mutations of the integrin β3 cytoplasmic tail in integrin αIIbβ3 bidirectional signaling using a trans-dominant inhibition model. Chimeric Tac-β3 proteins (i.e., Tac-β3, Tac-β3D741, Tac-β3D747, Tac-β3D754, Tac-β3D759, and Tac-β3DNITY) consisting of the extracellular and transmembrane domains of human IL-2 receptor (Tac) and the human integrin β3 cytoplasmic domain were stably expressed in the 123 CHO cells harboring human glycoprotein Ib-IX and wild-type integrin αIIbβ3. The different cells were assayed for stable adhesion and spreading on immobilized fibrinogen, and for binding soluble fibrinogen representing outside-in and inside-out signaling events, respectively. The chimeric protein Tac-β3 inhibited, and Tac-β3DNITY partially attenuated stable adhesion and spreading. Tac-β3, Tac-β3D759, Tac-β3DNITY, and Tac-β3D754, but not Tac-β3D747 or Tac-β3D741, impaired the soluble fibrinogen binding. Results indicated that the bidirectional signaling was significantly inhibited by Tac-β3 and Tac-β3DNITY, albeit to a much lesser extent. Moreover, only inside-out signaling was impaired in the 123/Tac-β3D759 and 123/Tac-β3D754 cells in contrast to an intact bidirectional signaling in the 123/Tac-β3D747 and 123/Tac-β3D741 cells. In conclusion, the calpain cleavage of integrin β3 resulted in the regulatory effects on signaling by interrupting its interaction with cytoplasmic proteins rather than altering its conformation, and may thus regulate platelet function.

关键词： integrin β3     signal transduction     trans-dominant inhibition model    

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Head-to-tail cyclization of a heptapeptide eliminates its cytotoxicity and significantly increases its inhibition

Shuai Ma, Huan Zhang, Xiaoyan Dong, Linling Yu, Jie Zheng, Yan Sun

《化学科学与工程前沿（英文）》 2018年 第12卷 第2期   页码 283-295 doi: 10.1007/s11705-017-1687-2

摘要： Amyloid- (A ) protein aggregation is the main hallmark of Alzheimer’s disease (AD). Inhibition of A fibrillation is thus a promising therapeutic approach to the prevention and treatment of AD. Recently, we designed a heptapeptide inhibitor, LVFFARK (LK7). LK7 shows a promising inhibitory capability on A fibrillation, but is prone to self-assembling and displays high cytotoxicity, which would hinder its practical application. Herein, we modified LK7 by a head-to-tail cyclization and obtained a cyclic LK7 (cLK7). cLK7 exhibits a different self-assembly behavior from LK7, and has higher stability against proteolysis than LK7 and little cytotoxicity to SH-SY5Y cells. Thermodynamic analysis revealed that both LK7 and cLK7 could bind to A by electrostatic interactions, hydrogen bonding and hydrophobic interactions, but the binding affinity of cLK7 for A ( = 4.96 µmol/L) is six times higher than that of LK7 ( = 32.2 µmol/L). The strong binding enables cLK7 to stabilize the secondary structure of A and potently inhibit its nucleation, fibrillation and cytotoxicity at extensive concentration range, whereas LK7 could only moderately inhibit A fibrillation and cytotoxicity at low concentrations. The findings indicate that the peptide cyclization is a promising approach to enhance the performance of peptide-based amyloid inhibitors.

关键词： Alzheimer’s disease     amyloid β-protein     cyclic peptide     inhibition     protein aggregation    

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冰灾对江西电网的灾害分析和电网应急平台体系建设

朱友梁,梁文,杨济海,陶振文,马勇,刘显明

《中国工程科学》 2010年 第12卷 第3期   页码 61-67

摘要：

回顾2009年全国大面积雪灾对江西省电网造成的巨大损失，分析了灾害的成因，并重点提出了电网应急管理平台建设的具体技术思路。

关键词： 电网     冰灾     应急    

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Effects of galectin-3 inhibition on endometrial cell cycle and adhesion

LEI Caixia, ZHANG Wei, SUN Xiaowei, DU Guoping, WANG Li, LIU Yinkun

《医学前沿（英文）》 2007年 第1卷 第4期   页码 390-397 doi: 10.1007/s11684-007-0076-5

摘要： Galectin-3 (gal-3) and its ligands have been implicated in cell transformation and cancer metastasis. Gal-3 protein has been found in uterine epithelial cells adjacent to implanting blastocysts in different cell types. In order to investigate the role of gal-3 in the establishment of human endometrial receptivity, the expression of gal-3 in human endometrial cell line RL95-2 was silenced by RNA interference technology using gal-3 specific small RNA. The expression of gal-3 was detected by the reverse transcriptase-polymerase chain reaction and Western blot analysis. After the suppression of gal-3, cell cycle changes and the expression of integrin 1 were detected by flow cytometry. The adhesive ability of RL95-2 cells was analyzed by the adhesion test. Gal-3 siRNA transfection efficiency reached 70%–90%. The expression of gal-3 mRNA and protein in RL95-2 cells was strongly inhibited by 70%–90% after RNA interference. Inhibition of gal-3 expression decreased S-phase but increased G1 phase cells. Integrin 1 expression was down-regulated, and the adhesive ability of RL95-2 cells to fibronectin (FN) was significantly reduced. Gal-3 may be involved in the establishment of endometrial receptivity by regulating the proliferation and adhesion of endometrial cells. The influence on adhesion may be related with the integrin modulation.
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Model test study for ice force on the bank-head of the lead-navigating bank

SONG An, SUN Jinliang, LU Hai, WANG Yi′an

《结构与土木工程前沿（英文）》 2007年 第1卷 第2期   页码 222-228 doi: 10.1007/s11709-007-0027-x

摘要： To determine the bank-head s ice force of the lead-navigating bank at key position that faces the ice directly of certain water conservancy, ice model test in reduced scale is designed and carried out based on the local situation on the spot and data observed. Preliminary conclusions are got through the analysis of the experimental data such as effect of the ice velocity on the ice force , ratio of components of forces when ice sheet acting on the vertical bank-head, ice force ratio of vertical to 45? incline as well as ratio of level ice force to the vertical ice force when acting on 45? incline. Finally, suggestions on determination of the ice force using formula are given after comparison between the result calculated by the present formula of ice force and the experimental results.

关键词： comparison     lead-navigating bank     vertical bank-head     certain     Preliminary    

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Comparison in executive function in Chinese preterm and full-term infants at eight months

null

《医学前沿（英文）》 2018年 第12卷 第2期   页码 164-173 doi: 10.1007/s11684-017-0540-9

摘要：

Executive function (EF) is increasingly recognized as being responsible for adverse developmental outcomes in preterm-born infants. Several perinatal factors may lead to poor EF development in infancy, and the deficits in EF can be identified in infants as young as eight months. A prospective cohort study was designed to study the EF in Chinese preterm infants and examine the relationship between EF in preterm infants and maternal factors during perinatal period. A total of 88 preterm infants and 88 full-term infants were followed from birth to eight months (corrected age). Cup Task and Planning Test was applied to assess the EF of infants, and the Bayley Scale of Infant Development (BSID-III) was used to evaluate cognitive (MDI) and motor abilities (PDI) of infants. In comparison with full-term infants, the preterm infants performed more poorly on all measures of EF including working memory, inhibition to prepotent responses, inhibition to distraction, and planning, and the differences remained after controlling the MDI and PDI. Anemia and selenium deficiency in mothers during pregnancy contributed to the differences in EF performance. However, maternal depression, hypertension, and diabetes during pregnancy were not related to the EF deficits in preterm infants. Future research should focus on the prevention of anemia and selenium deficiency during pregnancy and whether supplementing selenium in mothers during pregnancy can prevent further deterioration and the development of adverse outcomes of their offspring.

关键词： executive function (EF)     preterm infant     working memory     inhibition to prepotent response     inhibition to distraction     planning    

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标题 作者 时间 类型 操作

Rational Design of and Mechanism Insight into an Efficient Antifreeze Peptide for Cryopreservation

Haishan Qi,Yihang Gao,Lin Zhang,Zhongxin Cui,Xiaojie Sui,Jianfan Ma,Jing Yang,Zhiquan Shu,Lei Zhang,

期刊论文

Microstructure investigation of dynamic recrystallization in hard machining: From thermodynamic irreversibility

Binxun LI, Xinzhi ZHANG, Song ZHANG

期刊论文

Chronic inhibition of cyclic guanosine monophosphate-specific phosphodiesterase 5 prevented cardiac fibrosisthrough inhibition of transforming growth factor β-induced Smad signaling

null

期刊论文

Numerical simulation on flow of ice slurry in horizontal straight tube

Shengchun LIU,Ming SONG,Ling HAO,Pengxiao WANG

期刊论文

Application of Ice Pigging in a Drinking Water Distribution System: Impacts on Pipes and Bulk Water Quality

Yujing Huang,Zhiwei Chen,Guilin He,Yu Shao,Shuang Song,Feilong Dong,Tuqiao Zhang,

期刊论文

Purification of ice structuring protein complexes from winter wheat using Triton X-114 phase partitioning

Huaneng XU, Haiying CHEN, Weining HUANG,

期刊论文

simultaneous anammox and denitrification system with short-term pyridine exposure: Process capability, inhibition

期刊论文

ROOT EXUDATES FROM CANOLA EXHIBIT BIOLOGICAL NITRIFICATION INHIBITION AND ARE EFFECTIVE IN INHIBITING

期刊论文

Inhibition character of crotonaldehyde manufacture wastewater on biological acidification

期刊论文

Roles of integrin β3 cytoplasmic tail in bidirectional signal transduction in a trans-dominant inhibition

null

期刊论文

Head-to-tail cyclization of a heptapeptide eliminates its cytotoxicity and significantly increases its inhibition

Shuai Ma, Huan Zhang, Xiaoyan Dong, Linling Yu, Jie Zheng, Yan Sun

期刊论文

冰灾对江西电网的灾害分析和电网应急平台体系建设

朱友梁,梁文,杨济海,陶振文,马勇,刘显明

期刊论文

Effects of galectin-3 inhibition on endometrial cell cycle and adhesion

LEI Caixia, ZHANG Wei, SUN Xiaowei, DU Guoping, WANG Li, LIU Yinkun

期刊论文

Model test study for ice force on the bank-head of the lead-navigating bank

SONG An, SUN Jinliang, LU Hai, WANG Yi′an

期刊论文

Comparison in executive function in Chinese preterm and full-term infants at eight months

null

期刊论文